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. 2020 May 9;17:153. doi: 10.1186/s12974-020-01810-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Impact of RRMS sera treatment on the mitochondria content and membrane potential in brain ECs. (a, b) Representative flow cytometry plots and cumulative graphs of mitotracker green staining in hCMEC/D3 cells treated with 20% RRMS sera or HD for 16 h. (c, d) Representative flow cytometry plots and cumulative graphs of TMRE staining showing membrane potential in hCMEC/D3 treated with RRMS or HD sera. Data are expressed as mean ± s.e.m. of 30 donors/group (pooled in pairs matched in sex, age and EDSS score for RRMS patients). Statistical analysis by Mann-Whitney U test (two-tails) **P ≤ 0.01