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. 2020 Mar 10;28(5):1339–1358. doi: 10.1016/j.ymthe.2020.03.003

Figure 5.

Figure 5

Disruption of Glycine Decarboxylase (GLDC) Compromises the Functionality of Glycine in mdx Mice

(A) Western blot analysis to determine GLDC knockdown efficiency at the protein level in TA muscles transfected with GLDC shRNA-expressing AAV2/8 viruses in mdx mice 3 weeks later (n = 3). SC refers to AAV2/8 expressing scramble shRNA. (B) Immunohistochemistry for PAX7+ MuSCs in TA muscles from treated mdx mice (scale bar, 100 μm). The arrowheads point to PAX7+ MuSCs. (C) Quantitative analysis for PAX7+ MuSCs in TA muscles from treated mdx mice (n = 3). (D) Immunohistochemistry and quantitative analysis for eMyHC+ regenerating myofibers in TA muscles from treated mdx mice (n = 3) (scale bar, 100μm). (E) Immunohistochemistry for dystrophin-positive fibers in TA muscles from treated mdx mice (scale bar, 100 μm). (F) Western blot and quantitative analysis for dystrophin expression in TA muscles from treated mdx mice (n = 4). 2.5 and 5 μg of total protein from C57BL/6 mice and 50 μg of muscle samples from untreated and treated mdx mice were loaded. Two-tailed t test was used for statistical analysis. ∗p < 0.05, ∗∗p < 0.001. Data are presented as means ± SEM.