Figure 8.

Effects of Glycine on Cell Transplantation in mdx Mice

(A) Schematics for the cell transplantation in immunosuppressed mdx mice. i.v., intravenous injection; −7, 7 days prior to cell transplantation; −1, 1 day prior to cell transplantation. mdx mice were immunosuppressed during the experimental period unless otherwise specified. (B) Immunohistochemistry and quantitative analysis for dystrophin-positive fibers in TA muscles from glycine-treated mdx mice transplanted with wild-type MuSCs and muscles were harvested 3 weeks later (scale bar, 100 μm) (n = 4; two-tailed t test). (C) Measurement of TA muscle weight from mdx mice transplanted with MuSCs 3 weeks after transplantation. mdx refers to mdx controls without cell transplantation (n = 4). (D) Immunohistochemistry and quantitative analysis for dystrophin-positive fibers in TA muscles from glycine-treated mdx mice transplanted with wild-type primary myoblasts 3 weeks after transplantation (scale bar, 100 μm) (n = 4; two-tailed t test). (E) Tissue imaging to examine the GFP fluorescence and quantitative analysis of fluorescence intensity in glycine-treated mdx mice transplanted with wild-type GFP-positive MuSCs 3 weeks after transplantation (scale bar, 100 μm) (n = 6; one-way ANOVA and post hoc Student-Newman-Keuls test). (F and G) Immunohistochemistry (F) and quantitative analysis for dystrophin- and GFP-positive fibers (G) in TA muscles from glycine-treated mdx mice transplanted with GFP-positive MuSCs 3 weeks later (scale bar, 100 μm) (n = 6; two-tailed t test). Data are presented as means ± SEM. ∗p < 0.05, ∗∗p < 0.001.