Figure 4.

Topical Gentamicin Generated New Laminin 332 in GS-JEB Patients

(A and B) IF staining of skin biopsy specimens from PT1 (A), PT2 (B), and PT3 (B) taken prior to treatment and 1 month (1M) and 3 months (3M) after gentamicin treatment using monoclonal antibodies that specifically target the three chains (α3, β3, or γ2) of laminin 332 or the β4 integrin. Note that within the treated sites, gentamicin induced continuous expression of all three chains of laminin 332 at the DEJ of all three patients. Additionally, β4 integrin is increased and polarized along the entire DEJ after treatment. All images were obtained using the same camera and identical exposure times. Scale bars, 50 μm.