Figure 3.

Tumor Remission after ZIKV^BR Treatment in Dachshund Dog Bearing a Rare Intracranial Meningioma

(A) Representative dorsal and transversal tumor MRI at days 0, 14, 21, 42, 52, and 78 after clinical ZIKV^BR oncolytic treatment protocol onset. (B) Tumor volume growth kinetics quantified by MRI analysis. (C) Representative 3D MRI analyses of four different dimensions at day 0 and 14 after ZIKV^BR first injection. Scale bar, 1 cm. (D) Post-mortem macro images of CNS tumor. Scale bar, 1 cm. (E–G) Representative images of tumor positive for ZIKV^BR (red), microglia marker (G) (green), and nuclei DAPI (blue). Scale bar, 10 μm (E) and 20 μm (F and G). (H) H&E representative image of the tumor highlighting the lymphocytes infiltration (white arrowhead) and necrosis area with calcium accumulation (black arrow). Scale bar, 100 μm.