Table 1.
Tandem mass spectroscopy spectral counts of complement proteins, complement-regulating proteins, and Igs in membranous nephropathy
| Complement | Controls, average (±SD) n = 11 |
PLA2R, average (±SD) n = 7 |
EXT1/EXT2, average (±SD) n = 21 |
P value |
|---|---|---|---|---|
| C1qA | ND | 2 (±2) | 7 (±8) | 0.03 |
| C1qB | ND | 5 (±3) | 13 (±13) | 0.06 |
| C1qC | ND | 4 (±4) | 8 (±5) | 0.12 |
| C1r | ND | 8 (±6) | 18 (±35) | 0.59 |
| C1s | ND | 5 (±4) | 12 (±28) | 0.65 |
| C3 | 63 (±30) | 413 (±136) | 340 (±83) | 0.19 |
| C4A | 25 (±15) | 174 (±59) | 146 (±59) | 0.21 |
| C4B | 25 (±14) | 177 (±63) | 149 (±59) | 0.27 |
| C5 | 3 (±3) | 56 (±26) | 85 (±38) | 0.051 |
| C6 | 2 (±2) | 48 (±22) | 51 (±26) | 0.96 |
| C7 | 2 (±1) | 40 (±20) | 46 (±26) | 0.44 |
| C8α | 2 (±2) | 32 (±12) | 32 (±17) | 0.85 |
| C8β | ND | 26 (±8) | 30 (±18) | 0.59 |
| C8γ | ND | 19 (±10) | 20 (±10) | 0.63 |
| C9 | 9 (±5) | 79 (±32) | 82 (±51) | 0.59 |
| IgG1 | 31 (±44) | 68 (±12) | 97 (±36) | 0.06 |
| IgG2 | 26 (±12) | 49 (±7) | 75 (±29) | 0.03 |
| IgG3 | 25 (±11) | 64 (±18) | 74 (±30) | 0.49 |
| IgG4 | 13 (±7) | 91 (±28) | 71 (±35) | 0.13 |
| CD59 | 4 (±2) | 8 (±2) | 7 (±2) | 0.09 |
| CFB | 3 (±3) | 13 (±4) | 10 (±5) | 0.13 |
| CFD | ND | ND | ND | Not applicable |
| CFH | 10 (±6) | 123 (±40) | 87 (±23) | 0.03 |
| CFHR1 | 6 (±4) | 71 (±28) | 42 (±21) | 0.02 |
| CFHR2 | ND | 38 (±17) | 26 (±11) | 0.09 |
| CFHR3 | ND | 13 (±8) | 7 (±5) | 0.08 |
| CFHR4 | ND | 4 (±5) | 3 (±3) | 0.60 |
| CFHR5 | 4 (±3) | 101 (±46) | 61 (±22) | 0.008 |
| Clusterin | 14 (±6) | 53 (±12) | 46 (±26) | 0.07 |
| CR1 | 13 (±8) | 5 (±4) | 8 (±6) | 0.47 |
| Ficolin 3 | ND | ND | ND | Not applicable |
| Properdin | ND | 8 (±4) | 3 (±2) | 0.005 |
| Vitronectin | 21 (±6) | 73 (±29) | 55 (±29) | 0.04 |
EXT, exostosin; ND, none detected (spectral count of 1 or not detected); PLA2R, phospholipase A2 receptor.
Statistical significance (P value) is performed comparing PLA2R versus EXT1/EXT2-associated membranous nephropathy. The significant P values are highlighted in bold.