Figure 1.
Upregulation of circSKA3 in Human Breast Cancer
(A) Microarray showed increased expression of circSKA3 in breast cancer cell lines compared with normal breast cell line MCF-10A. (B) RT-PCR showed expression of circSKA3 in human non-tumor cell lines (HaCaT, 293T, Jurkat, and MCF10A), breast cancer cell lines (MCF-7, MDA-MB-468, MDA-MB-231, HTB126, and SK-BR-3), and other tumor cell lines (H460, LnCap, Du145, PC3, SNU449, HepG2, CI3K, A549, and HeLa). (C) Microarray showed increased expression of circSKA3 in human breast cancer compared with the adjacent benign tissues. (D) Breast cancer tissues expressed higher levels of circSKA3 than the adjacent benign tissues. (E) Correlation of breast cancer stages and circSKA3 levels was analyzed.