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. 2020 Jan 24;71(9):2612–2628. doi: 10.1093/jxb/eraa041

Fig. 10.

Fig. 10.

Schematic diagram showing the symplasmic domains found in different explants during somatic embryo culture. (A) IZE explants (representative for both WT and 35S:BBM IZEs) at the start and during the first day of culture; the entire explant is a single symplasmic domain for low-molecular-mass fluorochromes up to the sixth day of the culture. (B, C) Two symplasmic domains are detected for low-molecular-mass fluorochromes in WT IZE explants (B) and 35S:BBM IZE explants (C) between the sixth and eighth day of the culture, corresponding to an embryogenic region (red) and a non-embryogenic region (blue). (D) Symplasmic domains for 3 kDa tracers in 35S:BBM seedling explants. The three symplasmic domains are the non-embryogenic region (blue), the embryogenic cotyledon margin (yellow) in which 3 kDa dextran was retained, and the region with embryogenic protrusions (green), which is unable to take up 3 kDa dextran.