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. 2020 Jan 24;71(9):2612–2628. doi: 10.1093/jxb/eraa041

Fig. 9.

Fig. 9.

Callose deposition is associated with a decreased DR5v2 auxin response in embryogenic tissue. DEX-treated 35S:BBM-GR DR5v2:tdTomato explants were examined for DR5v2 expression (purple) and callose (blue) in control cultures (A–C) and DR5v2 expression (red) and callose (blue) in DDG-treated cultures (D). (A) Explants on day 4 of culture showing DR5 expression on the cotyledon surface. (B) Explant at day 6, showing reduced DR5v2 expression in patches along the cotyledon surface and margin. Callose begins to accumulate in areas with low DR5v2 expression. (C) More advanced explant at day 6 showing callose deposition in the cotyledon margin, distal to a region of reduced DR5v2 expression. (D) DR5v2 expression is maintained throughout the cotyledon surface after treatment with DDG for 6 d. (A–C) are CLSM and (D) epifluorescence microscopy images. Scale bars: (A–C) 50 µm; (D) 10 µm.