Table 1.

Quantification of dye movement between embryogenic and non-embryogenic regions after uncaging/application of fluorochromes and 3 kDa dextran in different Arabidopsis explants at 7 d after culture

Cell identity/ symplasmic domain	% movement from embryogenic to non-embryogenic areas			% movement from non-embryogenic to embryogenic areas
	CMNB (M=332 Da)	HPTS (M=520 Da)	Dextran (M=3 kDa)	CMNB (M=332 Da)	HPTS (M=520 Da)	Dextran (M=3 kDa)
WT IZEs on 2,4-D	4.9±11.3a (n=40)	2.7±7.6a (n=38)	0±0c (n=25)	5.5±7.6a (n=38)	0±0c (n=40)	0±0c (n=25)
35S:BBM IZEs	4.8±6.4a (n=42)	6.6±8.8a (n=27)	0±0c (n=19)	2.2±9.1a (n=41)	0±0c (n=32)	0±0c (n=19)
35S:BBM seedlings	100±0b (n=37)	100±0b (n=27)	2.8±7.6a (n=34)	100±0b (n=43)	100±0b (n=27)	0±0c (n=35)

The data are the mean ±SE of three biological replicates; n is the total number of areas where the tracer was uncaged/applied. % movement=(number of areas where the tracer moved/total number of areas where the tracer was uncaged/applied)×100; 0% movement indicates that cells following different developmental fates are symplasmically isolated; low % movement indicates very little movement/symplasmic communication between cells following different developmental fates; 100% movement indicates that cells following different developmental fates are highly symplasmically connected. A z-test for significance between percentage values was used to determine whether there are statistically significant differences between movement of tracers with different molecular masses between areas realizing different developmental programmes, within and between experimental systems (WT IZEs, 35S:BBM IZEs, and 35S:BBM seedlings). Each value was compared pairwise simultaneously and statistically significant differences (P<0.05) between values are indicated by different letters.