Fig. 4.
Oxygen accelerates PSII photoinhibition depending on LHCSR3 amounts. (A) Influence of high light (250 µmol photons m−2 s−1) on LHCSR3 levels in WT-D66, WT-4A, and npq4. High light-acclimated cultures were further high light treated for 1.5 h in the presence of 2.5 mM lincomycin and 5 mM NaHCO3 while purging with pure N2 (left panel; black) or pure O2 (right panel; white). PSII activity was measured after 1.5 h recovery by (B) O2 production at saturating light in the presence of 1 mM NaHCO3, and (C) Fv/Fm, n=3±SD. Data are from three independent experiments, with different letters denoting significant differences (P<0.05). The percentage changes from before to after high light treatment are indicated beneath (B) and (C).