Fig. 6.
High oxygen tensions during culturing lead to higher RES and other aldehyde levels, and more in npq4 than in the WT. Cells were grown photoautotrophically on 1.5% agar under photoautotrophic conditions in a 17% or 35% O2 atmosphere for 5 d under a 16/8 h light/dark cycle (250 µmol photons m−2 s−1). 2,4-DNPH-derivatized aldehydes, including several RES, were measured by LC-MS/MS, normalized to dry weight, and shown as fold change relative to the WT at 17% O2, as also indicated by shading. For each aldehyde, the P-values from a two-way ANOVA of genotype or percentage O2 are given in black (P<0.05) or grey (P>0.05), n=4±SD. (This figure is available in colour at JXB online.)