Fig. 1.

Identification by RNA-seq of differentially expressed genes (DEGs) responding to different treatments in post-harvest ‘Jingmianshi’ persimmon fruit. Mature fruit were treated with 95% CO₂ (CO₂), 95% CO₂+1 μl l^–1 1-methylcyclopropene (CO₂+1-MCP), or air (control, CK) for 1 d in air-tight containers, and then transferred to air. Samples were analysed 3 d after treatment. Orange indicates softening and yellow indicates that firmness was retained. The numbers of up- and down-regulated DEGs in each comparison are shown in the graphs. The Venn diagram to the left shows genes that had opposite trends in the two sets of comparisons. After removing the DEGs in the comparison between high-CO₂+1-MCP and CK, 227 DEGs remained that had maximum FPKM values ≥50.