Fig. 1.

De-esterification of HG in onion epidermal walls by PME. (A) Immunolabeling of the inner surface of onion epidermal cell walls after 2 h in HEPES buffer ±PME. JIM7 and LM19 antibodies detect HG of high and low esterifcation, respectively. Paired images ±PME were captured with identical light intensities and camera exposure settings. Scale bar=100 µm. Representative results from two independent replicates. (B) Methanol release from onion epidermal wall as a function of PME treatment time. Mean ±SE of two independent replicates.