Fig. 4.

Exposure to METH and/or IL-1β diminishes differentiation of neural progenitor cells (NPCs) to mature astrocytes and neurons. Primary mouse NPCs were induced to differentiate in the presence of METH (10 μM) and/or IL-1β (1 ng/ml) for 2 weeks. Control cells were exposed to vehicle. Then, the protein levels of GFAP and NeuN were assessed by immunoblotting as the markers of astrocytes and mature neurons, respectively. β-tubulin was assessed as the internal control. (A) Representative images, and (B and C) quantitative data from these experiments. The results were analyzed by one-way ANOVA with Tukey post hoc test; mean ± S.E. (n = 3 in each group; p < 0.05, **p < 0.01, ***p < 0.001 compared to control).