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. 2020 May 6;8:e9055. doi: 10.7717/peerj.9055

Table 2. Summary of the diversiy analysis of the extracellular proteases of the screened strains isolated from Scleractinian corals.

Genera Strains H/C ratioa Inhaibition ratiob(%)
Casein Gelatin Elastin PMSF
(1 mM)
OP
(1 mM)
E64
(0.1 mM)
P-A
(0.1 mM)
Vibrio sC18 4.20 5.16 0 54.57 49.26 26.43 19.43
sC57 2.30 2.41 4.00 81.86 6.60 27.74 18.21
D38 3.81 4.10 0 ND 64.20 ND ND
sL1 3.90 1.23 0 97.68 86.24 29.02 0
sL3 5.10 6.09 0 59.55 60.42 4.82 3.88
sQ3 1.88 3.56 1.41 91.09 87.88 2.67 −3.66
sQ48 2.16 3.18 1.53 98.56 81.12 10.25 16.02
Idiomarina yL5c 5.13 6.17 0 84.56 46.45 0 0
Pseudoalteromonas yL11 4.73 4.02 1.67 61.22 16.42 0 0
Shwanella yL13 2.86 2.55 1.54 88.03 −1.92 5.76 8.23
Pantoea sC6 2.16 2.02 0 65.2 92.47 6.12 −4.00
Microbulbifer sC1 4.1 4.22 0 93.01 67.83 10.53 23.32
sC9 3.78 2.16 0 91.78 75.26 9.25 −2.973
sC10 3.66 2.45 0 86.5 0 9.32 2.70
sD13 4.52 3.21 0 92.87 2.78 14.38 15.88
sD19 4.62 3.20 0 80.04 0 0 1.72
sL30 3.71 4.11 0 74.35 −3.52 6.33 8.76
Strenotrophomonas Q35 3.62 2.75 1.63 92.52 83.72 0.90 20.68
Pseudomonas sB27a 2.47 2.04 3.75 66.11 30.12 −6.12 0
sD40 3.20 2.81 2.36 91.30 82.74 0 −15.86
yQ15 4.00 3.42 1.26 92.50 78.14 1.20 6.46
Oceanicola sC16 3.60 3.25 1.58 63.14 51.51 17.27 27.77
Ruegeria sD55 3.02 3.56 2.36 53.03 57.04 12.23 24.30
Paracoccus yA2c 3.65 7.50 3.24 40.32 25.52 1.21 1.36
Sphingomonas sF30a 3.26 3.12 2.77 62.79 56.68 14.31 14.73
Pseudovibrio sC3 2.30 2.35 0 84.73 73.26 13.03 9.81
sC13 2.36 3.20 0 52.80 62.76 14.56 5.89
sC15 2.56 2.31 0 65.01 47.92 15.76 3.58
sL47 2.56 1.28 0 54.42 37.34 19.95 2.48
yF15 4.00 3.65 0 82.75 37.91 2.23 4.30
Micrococcus sF19 1.76 2.15 0 88.26 8.24 16.75 17.67
Fictibacillus sD46 1.62 2.81 2.45 90.23 56.42 28.56 8.48
sJ27 2.34 1.74 0 87.12 50.14 12.42 16.42
yQ18 1.88 2.01 2.01 84.12 40.13 8.45 3.88
Lysinibacillus B21 3.21 4.21 2.54 41.60 45.25 2.47 8.29
B24 3.02 4.05 3.21 65.21 42.00 −12.10 8.14
Bacillus sA10 1.64 5.21 0 92.85 2.48 15.88 33.29
sA50 2.32 4.00 2.65 85.30 76.40 24.59 25.39
yF12B 2.32 5.52 2.45 23.10 65.01 −1.25 6.24
sJ2 2.15 5.22 0 85.09 44.68 15.51 12.44
sJ36 1.33 1.42 0 90.20 −5.84 0 5.05
yL8 2.17 4.32 3.65 89.32 5.03 13.02 0
yL12 2.36 2.30 1.96 86.20 44.52 12.34 0
yQ16 3.02 5.25 5.63 56.56 43.22 0 0
Aquimarina sC2 3.60 4.32 0 90.00 12.15 12.34 35.02
sC26 2.46 3.30 2.26 82.07 0 5.76 8.23
sJ58 3.01 3.65 0 77.62 10.31 23.72 11.44

Notes.

a

H/C ratio is the ratio of the hydrolytic zone diameter vs. the colony diameter of a colony on the plate.

a

Inhibition ratio (%) was calculated by using control activity minus the relative activity of a sample with an inhibitor and the activity of a sample without any inhibitor was taken as a control (%).

PMSF
phenylmethylsulfonyl fluoride
OP 1
10-phenanthroline
P-A
pepstatin A

Pocillopora, Porites, Platygyra, Turbinaria, Faviia, Acropora, Montipora were represented by A, B, C, D, F, L, Q.