Table 2. Summary of the diversiy analysis of the extracellular proteases of the screened strains isolated from Scleractinian corals.
| Genera | Strains | H/C ratioa | Inhaibition ratiob(%) | |||||
|---|---|---|---|---|---|---|---|---|
| Casein | Gelatin | Elastin |
PMSF (1 mM) |
OP (1 mM) |
E64 (0.1 mM) |
P-A (0.1 mM) |
||
| Vibrio | sC18 | 4.20 | 5.16 | 0 | 54.57 | 49.26 | 26.43 | 19.43 |
| sC57 | 2.30 | 2.41 | 4.00 | 81.86 | 6.60 | 27.74 | 18.21 | |
| D38 | 3.81 | 4.10 | 0 | ND | 64.20 | ND | ND | |
| sL1 | 3.90 | 1.23 | 0 | 97.68 | 86.24 | 29.02 | 0 | |
| sL3 | 5.10 | 6.09 | 0 | 59.55 | 60.42 | 4.82 | 3.88 | |
| sQ3 | 1.88 | 3.56 | 1.41 | 91.09 | 87.88 | 2.67 | −3.66 | |
| sQ48 | 2.16 | 3.18 | 1.53 | 98.56 | 81.12 | 10.25 | 16.02 | |
| Idiomarina | yL5c | 5.13 | 6.17 | 0 | 84.56 | 46.45 | 0 | 0 |
| Pseudoalteromonas | yL11 | 4.73 | 4.02 | 1.67 | 61.22 | 16.42 | 0 | 0 |
| Shwanella | yL13 | 2.86 | 2.55 | 1.54 | 88.03 | −1.92 | 5.76 | 8.23 |
| Pantoea | sC6 | 2.16 | 2.02 | 0 | 65.2 | 92.47 | 6.12 | −4.00 |
| Microbulbifer | sC1 | 4.1 | 4.22 | 0 | 93.01 | 67.83 | 10.53 | 23.32 |
| sC9 | 3.78 | 2.16 | 0 | 91.78 | 75.26 | 9.25 | −2.973 | |
| sC10 | 3.66 | 2.45 | 0 | 86.5 | 0 | 9.32 | 2.70 | |
| sD13 | 4.52 | 3.21 | 0 | 92.87 | 2.78 | 14.38 | 15.88 | |
| sD19 | 4.62 | 3.20 | 0 | 80.04 | 0 | 0 | 1.72 | |
| sL30 | 3.71 | 4.11 | 0 | 74.35 | −3.52 | 6.33 | 8.76 | |
| Strenotrophomonas | Q35 | 3.62 | 2.75 | 1.63 | 92.52 | 83.72 | 0.90 | 20.68 |
| Pseudomonas | sB27a | 2.47 | 2.04 | 3.75 | 66.11 | 30.12 | −6.12 | 0 |
| sD40 | 3.20 | 2.81 | 2.36 | 91.30 | 82.74 | 0 | −15.86 | |
| yQ15 | 4.00 | 3.42 | 1.26 | 92.50 | 78.14 | 1.20 | 6.46 | |
| Oceanicola | sC16 | 3.60 | 3.25 | 1.58 | 63.14 | 51.51 | 17.27 | 27.77 |
| Ruegeria | sD55 | 3.02 | 3.56 | 2.36 | 53.03 | 57.04 | 12.23 | 24.30 |
| Paracoccus | yA2c | 3.65 | 7.50 | 3.24 | 40.32 | 25.52 | 1.21 | 1.36 |
| Sphingomonas | sF30a | 3.26 | 3.12 | 2.77 | 62.79 | 56.68 | 14.31 | 14.73 |
| Pseudovibrio | sC3 | 2.30 | 2.35 | 0 | 84.73 | 73.26 | 13.03 | 9.81 |
| sC13 | 2.36 | 3.20 | 0 | 52.80 | 62.76 | 14.56 | 5.89 | |
| sC15 | 2.56 | 2.31 | 0 | 65.01 | 47.92 | 15.76 | 3.58 | |
| sL47 | 2.56 | 1.28 | 0 | 54.42 | 37.34 | 19.95 | 2.48 | |
| yF15 | 4.00 | 3.65 | 0 | 82.75 | 37.91 | 2.23 | 4.30 | |
| Micrococcus | sF19 | 1.76 | 2.15 | 0 | 88.26 | 8.24 | 16.75 | 17.67 |
| Fictibacillus | sD46 | 1.62 | 2.81 | 2.45 | 90.23 | 56.42 | 28.56 | 8.48 |
| sJ27 | 2.34 | 1.74 | 0 | 87.12 | 50.14 | 12.42 | 16.42 | |
| yQ18 | 1.88 | 2.01 | 2.01 | 84.12 | 40.13 | 8.45 | 3.88 | |
| Lysinibacillus | B21 | 3.21 | 4.21 | 2.54 | 41.60 | 45.25 | 2.47 | 8.29 |
| B24 | 3.02 | 4.05 | 3.21 | 65.21 | 42.00 | −12.10 | 8.14 | |
| Bacillus | sA10 | 1.64 | 5.21 | 0 | 92.85 | 2.48 | 15.88 | 33.29 |
| sA50 | 2.32 | 4.00 | 2.65 | 85.30 | 76.40 | 24.59 | 25.39 | |
| yF12B | 2.32 | 5.52 | 2.45 | 23.10 | 65.01 | −1.25 | 6.24 | |
| sJ2 | 2.15 | 5.22 | 0 | 85.09 | 44.68 | 15.51 | 12.44 | |
| sJ36 | 1.33 | 1.42 | 0 | 90.20 | −5.84 | 0 | 5.05 | |
| yL8 | 2.17 | 4.32 | 3.65 | 89.32 | 5.03 | 13.02 | 0 | |
| yL12 | 2.36 | 2.30 | 1.96 | 86.20 | 44.52 | 12.34 | 0 | |
| yQ16 | 3.02 | 5.25 | 5.63 | 56.56 | 43.22 | 0 | 0 | |
| Aquimarina | sC2 | 3.60 | 4.32 | 0 | 90.00 | 12.15 | 12.34 | 35.02 |
| sC26 | 2.46 | 3.30 | 2.26 | 82.07 | 0 | 5.76 | 8.23 | |
| sJ58 | 3.01 | 3.65 | 0 | 77.62 | 10.31 | 23.72 | 11.44 | |
Notes.
H/C ratio is the ratio of the hydrolytic zone diameter vs. the colony diameter of a colony on the plate.
Inhibition ratio (%) was calculated by using control activity minus the relative activity of a sample with an inhibitor and the activity of a sample without any inhibitor was taken as a control (%).
- PMSF
- phenylmethylsulfonyl fluoride
- OP 1
- 10-phenanthroline
- P-A
- pepstatin A
Pocillopora, Porites, Platygyra, Turbinaria, Faviia, Acropora, Montipora were represented by A, B, C, D, F, L, Q.