KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-GFP rabbit polyclonal antibody | ThermoFisher Scientific | A-11122, RRID:AB_221569 |
| anti-brp mouse monoclonal antibody | Developmental Studies Hybridoma Bank | nc82, RRID:AB_2314866 |
| anti-DsRed rabbit polyclonal antibody | Takara | Cat # 632496; RRID:AB_10013483 |
| anti-GFP chicken polyclonal antibody | abcam | Cat# ab13970; RRID:AB_300798 |
| anti-HA rabbit monoclonal antibody | Cell Signaling Technologies | Cat# 3724S; RRID:AB_1549585 |
| anti-FLAG rat monoclonal antibody | Novus Biologicals | Cat# NBP1-06712; RRID: AB_1625981 |
| anti-DyLight549-conjugated V5 mouse monoclonal antibody | AbD Serotec | Cat# MCA1360D549; RRID:AB_915420 |
| Alexa Fluor 488, donkey anti-mouse | Jackson Immuno Research Lab | Cat# 715-545-151; RRID: AB_2341099 |
| Alexa Fluor 488, goat anti-chicken | abcam | Cat# ab150169; RRID:AB_2636803 |
| Alexa Fluor 488, goat anti-rabbit | ThermoFisher Scientific | Cat# A-11008; RRID:AB_143165 |
| Alexa Fluor 647, goat anti-mouse | ThermoFisher Scientific | Cat# A-21236; RRID:AB_141725 |
| Alexa Fluor 647, donkey anti-rat | Jackson Immuno Research Lab | Cat# 712-605-153; RRID: AB_2340694 |
| Alexa Fluor 568, goat anti-mouse | ThermoFisher Scientific | Cat# A-11004 RRID:AB_141371 |
| Alexa Fluor 568, goat anti-rabbit | ThermoFisher Scientific | Cat# A-11036; RRID:AB_10563566 |
| Alexa Fluor 594, donkey anti-rabbit | Jackson Immuno Research Lab | Cat# 711-585-152; RRID: AB_2340621 |
| anti-GABA rabbit polyclonal antibody | Sigma-Aldrich | Cat# A2052; RRID:AB_477652 |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: LC11-SplitGal4 [R22H02-p65.AD(attP40); R20G06-Gal4.DBD(attP2)] | Bloomington | RRID:BDSC_68362 |
| D. melanogaster: LC11-LexA [R22H02-LexA(attP40)] | Bloomington | RRID:BDSC_52699 |
| D. melanogaster: LC11-LexA [R20G06-LexA(attP40)] | Bloomington | RRID:BDSC_52571 |
| D. melanogaster: LC11-LexA [R51F09-LexA(attP40)] | Bloomington | RRID:BDSC_54884 |
| D. melanogaster: T4/T5-SplitGal4 [R59E08-p65.AD(attP40); R42F06-Gal4.DBD(attP2)] | JRC, HHMI, G. Rubin | N/A |
| D. melanogaster: T2-Gal4 [R47E02-Gal4(attP2)] | Bloomington | RRID:BDSC_50309 |
| D. melanogaster: T2-Gal4 [VT012791-Gal4(attP2)] | JRC, HHMI, B. Dickson | N/A |
| D. melanogaster: T2-SplitGal4 [VT012791-p65ADZp(attP40); R47E02- ZpGDBD(attP2)] | This Study | N/A |
| D. melanogaster: T3-SplitGal4 [VT002055-p65ADZp(attP40); R65B04- ZpGDBD(attP2)] | This Study | N/A |
| D. melanogaster: T3-Gal4 [VT002055-Gal4(attP2)] | JRC, HHMI, B. Dickson | N/A |
| D. melanogaster: T3-Gal4 [VT008463-Gal4(attP2)] | JRC, HHMI, B. Dickson | N/A |
| D. melanogaster: empty-split Gal4 [BPp65ADZp(attP40); BPZpGdbd(attP2)] | Bloomington | RRID:BDSC_79603 |
| D. melanogaster: T2/T3-Gal4 [R65B04-Gal4(attP2)] | Bloomington | RRID:BDSC_39336 |
| D. melanogaster: Tm3-Gal4 [R13E12-Gal4(attP2)] | Bloomington | RRID:BDSC_48569 |
| D. melanogaster: Tm4-Gal4 [R35H01-Gal4(attP2)] | Bloomington | RRID:BDSC_49922 |
| D. melanogaster: Tm9-Gal4 [0137-Gal4] | Bloomington | RRID:BDSC_62661 |
| D. melanogaster: Tm2-Gal4 [VT12282-Gal4 (attP2] | VDRC | 203097 |
| D. melanogaster: 13xLexAop2-IVS-GCaMP6f-p10(su(Hw)attP5) | Bloomington | RRID:BDSC_44277 |
| D. melanogaster: 20xUAS-IVS-GCaMP6f(attP40) | Bloomington | RRID:BDSC_42747 |
| D. melanogaster: 8xLexAop2-FLPL(attP40) | Bloomington | RRID:BDSC_55820 |
| D. melanogaster:10xUAS-IVS-mCD8::RFP(attP18),13LexAop2-IVS-mCD8::GFP(su(Hw)attP8 | Bloomington | RRID:BDSC_32229 |
| D. melanogaster: UAS-myrGFP.QUAS-mtdTomato-3xHA(su(Hw)attP8); trans-TANGO (attP40) | Bloomington | RRID:BDSC_77124 |
| D. melanogaster: Mi{FlpStop}Rdl[MI02620-FlpStop.ND] | Bloomington | RRID:BDSC_67682 |
| D. melanogaster: Mi{Trojan-Gal4.1}Rdl[MI02957-TG4.1] | Bloomington | RRID:BDSC_65421 |
| D. melanogaster: Mi{Trojan-GAL4.0}nAChRalpha1[MI00453-TG4.0] | Bloomington | RRID:BDSC_66780 |
| D. melanogaster: Mi{Trojan-GAL4.2}nAChRalpha5[MI13859-TG4.2] | Bloomington | RRID:BDSC_76755 |
| D. melanogaster: Mi{Trojan-GAL4.1}nAChRalpha6[MI01466-TG4.1] | Bloomington | RRID:BDSC_76137 |
| D. melanogaster: Mi{Trojan-GAL4.1}nAChRalpha7[MI12545-TG4.1] | Bloomington | RRID:BDSC_77828 |
| D. melanogaster: Mi{Trojan-GAL4.0}ChAT[MI04508-TG4.0] | Bloomington | RRID:BDSC_60317 |
| D. melanogaster: Mi{Trojan-GAL4.2}VGlut[MI04979-TG4.2] | Bloomington | RRID:BDSC_60312 |
| D. melanogaster: pBPhsFlp2::PEST (attP3); pJFRC201-10XUAS-FRT > STOP > FRT-myr::smGFP-HA (VK0005), pJFRC240-10XUAS-FRT > STOP > FRT-myr::smGFP-V5-THS-10XUAS-FRT > STOP > FRT-myr::smGFP-FLAG (su(Hw)attP1) | Bloomington | RRID:BDSC_64085 |
| D. melanogaster: 10XUAS-Syn21-Chrimson88-tdT-3.1 (attP18) | JRC, HHMI, M. Reiser, Strother et al., 2017 | N/A |
| D. melanogaster: LexAop2-Syn21-opGCaMP6s (su(Hw)attP8) | JRC, HHMI, M. Reiser, Strother et al., 2017 | N/A |
| D. melanogaster: 20XUAS-TTS-Shibire-ts1-p10 (VK0005) | JRC, HHMI, G. Rubin, Pfeiffer et al., 2012 | N/A |
| Software and Algorithms | ||
| MATLAB R2016a | MathWorks | https://www.mathworks.com/ |
| Affinity Designer | Serif | https://www.serif.com/en-us/ |
| Fiji | http://fiji.sc/ | RRID: SCR_002285 |
| Slidebook | https://www.intelligent-imaging.com/slidebook | SlideBook , RRID:SCR_014300 |