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. 2020 Apr 21;52(4):672–681. doi: 10.1038/s12276-020-0424-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b mRNA and protein expression of Sox9 in ASCs and chondrocytes. Normalized by GAPDH and B-actin. *p < 0.05 compared to ASCs **p < 0.001 compared to ASCs. n = 3. c ASCs were transfected with 50 nM Sox9 siRNA for 24 h and cultured in chondrocyte differentiation medium (DM). The cells were transfected with the Sox9 siRNA every 3 days for 16 days while cultured in DM. The mRNA expression of Sox9, Aggrecan, and Type II collagen in the Sox9 siRNA-transfected ASCs. C and Cont control, R reagent-transfected ASCs, siR Sox9 siRNA-transfected ASCs, DM chondrogenic differentiation induction medium. *p < 0.05, **p < 0.001 compared to the control, ^#p < 0.05, ^##p < 0.001 compared to the reagent-transfected and DM-treated ASCs. n = 3.