Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 21;52(4):672–681. doi: 10.1038/s12276-020-0424-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Structure of ellipticine. b Concentration-dependent cell viability was measured by cell viability assays in ASCs. D DMSO-treated ASCs *p < 0.05, ***p < 0.001 compared to the control. n = 3. c The ability of ELPC to induce differentiation was confirmed using Alcian blue staining after ASCs were incubated with ELPC for 16 days. Scale bar: 3 mm. d A 3D pellet culture was performed using ASCs or ELPC-treated ASCs for 16 days. **p < 0.01 compared to the control. n = 3. e The untreated ASC and ELPC-treated ASC pellets were stained using Alcian blue and H&E. The expression of Type II collagen, Aggrecan, and Type X collagen was detected by immunofluorescence. Nuclei were stained with DAPI (Blue). Scale bar: upper 400 μm and lower 50 μm. f The mRNA expression of the extracellular matrix degradation enzymes ADAMTS4, ADAMTS5, and MMP13. Normalized by GAPDH. *p < 0.05, ***p < 0.001 compared to the control. n = 3. g The concentrations of secreted ADAMTS4 and ADAMTS5 were measured by ELISAs. ***p < 0.001 compared to the control, ^#p < 0.001 compared to the 16-day control, ^$p < 0.001 compared to the ELPC-treated ASCs. C and Cont control, DM chondrogenic differentiation induction medium, ELPC ELPC-treated ASCs. n = 3.