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. 2020 May 9;12:14. doi: 10.1038/s41368-020-0077-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Nron negatively correlates with osteoclast activity in alveolar bone. a RT-qPCR analysis of NRON expression in alveolar bone specimens from young and aged individuals; n = 8 per group. b RT-qPCR analysis of Nron expression in alveolar bone specimens from 2-month-old and 12-month-old WT mice; n = 8 per group. c RT-qPCR analysis of Nron expression in BMMs, OCs, BMSCs and OBs from 2-month-old WT mice. BMMs, bone marrow-derived macrophages; OCs, osteoclasts; BMSCs, bone marrow stromal cells; OBs, osteoblasts. n = 8 per group. d RT-qPCR analysis of Nron expression in BMMs from 2-month-old WT mice during osteoclast differentiation; n = 6 per group. e RT-qPCR analysis of RANKL/OPG expression in alveolar bone specimens from young and aged individuals; n = 8 per group. f RT-qPCR analysis of Rankl/Opg expression in alveolar bone specimens from 2-month-old and 12-month-old WT mice; n = 6 per group. RT-qPCR quantitative reverse transcription polymerase chain reaction. GAPDH and Gapdh were used as endogenous controls in RT-qPCR analysis of human and mouse specimens, respectively. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1