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. 2020 May 9;12:15. doi: 10.1038/s41368-020-0079-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Biofilm architecture and EPS distribution of S. epidermidis strains observed by confocal microscopy. a Double-labeling of 24 h S. epidermidis biofilms. Green, bacteria (SYTO 9); red, EPS (Alexa Fluor 647). The three-dimensional reconstruction of the biofilms and the quantification of bacteria/EPS biomass were both performed with IMARIS 7.0.0. b The distributions of EPS and bacteria at different heights. c Quantification of bacteria/EPS biomass was performed with IMARIS 7.0.0. Results are the average of five randomly selected positions of each sample and are presented as mean ± standard deviation. P < 0.05. d The ratio of EPS to bacteria at different heights was quantified with IMARIS 7.0.0. Results are the average of five randomly selected positions of each sample and are presented as mean ± standard deviation. EPS, Extracellular Polymeric Substances