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. 2020 May 8;11:2271. doi: 10.1038/s41467-020-16107-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Table of TFs that have been implicated to interact with Tup1–Cyc8 and have motif sequences within the IME1 promoter. BS = binding site. b Multiple TFs associate with the IME1 promoter in the same region where Tup1–Cyc8 binds. TFs listed in a were tagged with V5 epitope tag. Diploid cells harbouring V5-tagged transcription factor (YAP6-V5, FW3833; SOK2-V5, FW5638; PHD1-V5, FW4466; SUT1-V5, FW6974; MOT3-V5, FW4383; SKO1-V5, FW4389; NRG1-V5, FW4393; NRG2-V5, FW4396; SKN7-V5, FW4399; CIN5-V5, FW7072; SFL1-V5, FW7070; MIG1-V5, FW4665; RGT1-V5, FW4386) and control cells (untagged, FW1511) were grown till exponential growth. The region around 1000 bp upstream of IME1 AUG was analysed for binding by ChIP. The signals were normalised over HMR. Red bars represent a signal of three-fold over HMR or more. Mean and SEM of n = 4 (Yap6-V5 and Sut1-V5), n = 3 (Sok2-V5 and Phd1-V5) and n = 2 for other TFs plus untagged control are shown. c Similar as b, except that TFs that showed at least three-fold enrichment in b were analysed for binding throughout the whole IME1 promoter. Eight primer pairs were used for the analyses. The mean of n = 2 is shown.