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. 2020 May 8;10:7768. doi: 10.1038/s41598-020-64710-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Specificity, and sensitivity determined by (B) visual color and (C) spectrophotometry of LAMP-AuNP. In A, tube N represents no template control; 1, S. aureus ATCC13565; 2, S. aureus ATCC 25923; 3, S. aureus ATCC25928; 4, S. aureus ATCC144925; 5, S. epidermidis ATCC12228; 6, S. saprophyticus ATCC15305; 7, V. funissii; 8, S. flexneri; 9, E. coli ATCC25922; 10, E. coli ATCC35218; 11, S. typhimurium ATCC14026; 12, E. cloacae; 13, A. sobria; 14, E. faecalis ATCC25912. In B and C, S. aureus ATCC13565 DNA was as template; lane 1, 9.7 ng/µL; 2, 0.97 ng/µL; 3, 0.097 ng/µL; 4, 9.7 pg/µL; 5, 0.97 pg/µL; 6, 0.097 pg/µL; 7, 9.7 fg/µL; 8, 0.97 fg/µL.