Table 2.
Sensitivity comparison between the culture method by ISO/IEC 17025 laboratory (10–105 CFU per g of ground pork, or mL of milk) and our crude DNA lysis coupled LAMP-AuNP (1–105 CFU per g of ground pork, or mL of milk) using live S. aureus ATCC13565 culture spike.
| Pork (CFU/g) | Culture (CFU/g) | LAMP-AuNP | Milk (CFU/mL) | Culture (CFU/mL) | LAMP-AuNP |
|---|---|---|---|---|---|
| 105 | + (3,000,000) | + | 105 | + (360,000) | + |
| 104 | + (320,000) | + | 104 | + (34,000) | + |
| 103 | +/− (<10) | + | 103 | + (1,900) | + |
| 102 | +/− (<10) | + | 102 | + (235) | + |
| 10 | +/− (<10) | + | 10 | + (20) | + |
| 1 | N/A* | − | 1 | N/A | − |
N/A represents data not available, because 1 CFU spiked-in experiment was not performed for the culture method.