Fig. 3.

DHM administration counteracts EtOH‐mediated inhibition of AMPK and downstream lipid metabolic responses. Representative WB of phosphorylated AMPK (p‐AMPK [Thr172]), total AMPK, phosphorylated ACC1 (p‐ACC1 [Ser79]), total ACC1, total CPT1a, and β‐actin loading control. EtOH‐fed mice showed a significant reduction in p‐AMPK (Thr172) and increase in p‐ACC1 (Ser79) relative to water‐fed controls (Ctl). DHM administration at both 5 and 10 mg/kg resulted in a significant increase in p‐AMPK (Thr172) and CPT1a expression, and a significant reduction in p‐ACC1 (Ser79) relative to EtOH‐fed mice and water‐fed mice (Ctl). The Western blot images are representative of Western blots obtained from 3 different biological experiments. n = 3/group. See Fig. S3 for ImageJ quantification of triplicates.