Fig. 7.

DHM increases the expression of catalase and suppresses ethanol (EtOH)‐mediated ROS generation in vitro. (A) Representative Western blot image of HepG2 and VL‐17A cells cultured in EtOH and treated with  5 µM DHM or untreated for 24 hours and immunoblotted with anti‐Catalase mAb. (B) HepG2 and VL‐17A cells were cultured in 50 to 100 mM EtOH and treated with 2.5 to 5 µM DHM or untreated for 24 hours before fluorometric analysis of intracellular ROS levels. Bar graphs were generated by quantifying blots from 3 independent experiments using ImageJ and normalized against intensity of the untreated lane. Data represented as mean ± SEM. *p < 0.05 and **p < 0.01 compared with corresponding EtOH controls and normalized with untreated controls; †p < 0.05 versus untreated control. n = 3/group. ROS, reactive oxygen species. A.U., arbitrary units.