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. 2020 Apr 6;16(11):1861–1875. doi: 10.7150/ijbs.45112

Figure 2.

Figure 2

Osblr8 is a pluripotency-associated lncRNA. A) Differential expression of Osblr8 in reprogramming. Fib: fibroblasts; non-iPSC: unreprogrammed cells that express four OSKM cocktail factors but failed to complete reprogramming; iPSC: induced pluripotent stem cells; E14: mouse pluripotent stem cell line used as a positive control. Gene expression was measured by Q-PCR and normalized to β-Actin. For comparison, E14 embryonic pluripotent stem cells were used as the positive control. B) The expression of Osblr8 is associated with Oct4, Sox2, and Nanog expression in embryoid body (EB) differentiation. iPSCs were collected at different stages of EB differentiation for quantitative PCR. C) Subcellular localization of Osblr8 lncRNA. RNA extracted from each fraction was analyzed by Q-PCR. Data shown are mean ± SD (n = 3). β-Actin was used as the cytoplasmic control and U6 was used as the nuclear control. D) RNA FISH of Osblr8. LncRNA probes were synthesized using DIG-11-dUTP and detected by anti-digoxigenin-fluorescein (green). DAPI was used to stain the nucleus of iPSCs (blue). Osblr8 lncRNA was predominantly located in the nucleus.