Figure 4.

Osblr8 activates stem cell core factor genes. A) Lentiviral Osblr8 overexpression in fibroblasts. Vector: empty lentiviral vector; lncCT: lncRNA random control; Osblr8: Lentiviral Osblr8 overexpression. ** p<0.001 as compared with the Vector and lncRNA controls. B) Lentiviral overexpression of Osblr8 activates the endogenous stem cell core factor genes. *** p<0.0001, ** p<0.001 as compared with the Vector and lncRNA controls. C) Osblr8 activates promoter activities of core pluripotent factors. 293T cells were co-transfected by reporter plasmids and Osblr8 plasmid. Forty-eight hours after transfection, cells were collected for luciferase activity measurements. Reporter plasmid empty vector and random lncRNA (lncCT) vectors were used as the controls. For comparison, the untreated fibroblasts were set as 1. * P < 0.05, and ** P < 0.001.