Figure 5.

Osblr8 interacts with TET protein family. A) Knockdown of Osblr8 in iPSCs. B) TET family expression in Osblr8 knockdown iPSCs. cDNAs were extracted from iPSCs loss-of-function experiment. The TET1/2/3 primers were used for Q-PCR. C) Interaction of Osblr8 with TET enzymes by RNA-chromatin immunoprecipitation (RIP). The TET-RNA chromatin complex was immunoprecipitated with an antibody against TET1, TET2 and TET3. After de-crosslinking, the immunoprecipitated RNAs were reverse transcribed. The TET-interacting Osblr8 was measured by Q-PCR. IgG was use as the antibody control. Input: aliquot DNAs collected during the RIP assay. * P < 0.05, ** P < 0.01, and *** P < 0.001. NS: not significant.