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. 2020 Apr 27;16(11):1989–2000. doi: 10.7150/ijbs.43062

Figure 3.

Figure 3

The effects of MYLK-AS1 on HCC cell migration and invasion in vitro. (A) BEL-7402 and MHCC97-H cells were transfected with MYLK-AS1 siRNAs to knockdown the expression of MYLK-AS1. Wound healing assay was performed to evaluate cell migration changes between control and MYLK-AS1 knockdown groups in BEL-7402 and MHCC97-H cells. (B) Cells were transfected as in (A). Transwell assay was performed to investigate cell invasion differences between control and MYLK-AS1 knockdown groups in BEL-7402 and MHCC97-H cells. (C and D) HepG2 cells were transfected with pcDNA3.0-MYLK-AS1 to overexpress MYLK-AS1. Wound healing assay and transwell assay were respectively used to detect cell migration (C) and invasion (D) differences between control and MYLK-AS1 overexpression groups in HepG2 cells. Data shown are mean ± SD of 3 independent experiments. (*P < 0.05 versus empty vector or control siRNA, ** P < 0.01 versus empty vector or control siRNA).