Figure 11.

Effect of silibinin on H/R-activated inflammatory response and NF-κB signaling in H9C2 cells. A, IL-6 and TNF-α transcription levels determined by real-time PCR in H9C2 cells exposed to H/R (n=4 for each). B, IL-6 and TNF-α levels determined by ELISA in cultured medium after H/R (n=4 for each). C, Representative WB and quantitative analysis of IκBα, p-NF-κB, NF-κB, p65 NF-κB and p-IKKα in reperfused myocardium (n=4 for each). *p<0.05 vs. Sham+vehicle. ^#p<0.05 vs. H/R+vehicle. H/R, hypoxia/reperfusion.