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. 2020 Apr 27;16(11):1972–1988. doi: 10.7150/ijbs.39259

Figure 2.

Figure 2

Silibinin treatment limits infarct size, reduced cardiomyocytes apoptosis after I/R injury. A, Representative images of transverse heart sections after Evans Blue and TTC double staining. B, Quantitative analysis of infarct area and AAR at 24 hours after I/R (n=6 for each). C, Representative immunofluorescences of TUNEL (green), α-actinin (red), and DAPI (blue) staining in the infarct border zone. D, Quantitative analysis of TUNEL-positive cardiomyocytes in the infarct border zone at 24 hours after I/R (n=6 for each). E, Measurement of caspase-3 activity in reperfused myocardium at 24 hours after I/R (n=6 for each). F and G, Bcl-2 and Bax transcription levels determined by real-time PCR (n=6 for each). Bar=50 μm. *p<0.05 vs. Sham+vehicle. #p<0.05 vs. I/R+vehicle. NS, not significant.