Fig. 6.

The ability of compound 22 to induce apoptosis in A375 cells. Briefly, cells were exposed to 100 μM of compound 22 at 24, 48 and 72 h and then the number of live, apoptotic and necrotic cells were recorded by means of (a) flow cytometry and also quantified as (b) percent of total cell population. Data shown are means ± SD of 3 replicates from three independent experiments. Asterisks (**) and (***) denote statistical significance at p < 0.01 and p < 0.001 respectively when compared to their respective control (untreated cells); (c) The ability of compound 22 to induce the expression of intrinsic and extrinsic apoptotic markers in A375 cells. Cells were subjected to 100 μM of compound 22 for 24, 48 and 72 h and protein expression levels of full length and cleaved caspases-8 and -9 were recorded in addition to those of BID and Apaf-1