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. 2020 Apr 22;22(4):502–513. doi: 10.22074/cellj.2021.6917

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Fig.5 — Cytokine production of anti-MUC1 chimeric antigen receptor (CAR)-T cells. Anti-MUC1 CAR-T cells or untransduced T cells co-cultured for 72 hours with MUC1-positive cells (T47D and MCF-7) or MUC1-negative cells (A431). A-C. Concentrations of IL-2, D-F. TNF alpha and G-I. IFN-γ secreted into supernatants was quantified by the enzyme-linked immunosorbent assay (ELISA). A single culture from each of cell lines was maintained as the negative control. The results are shown as the mean ± SD of three samples (n=3). *; P=0.033.