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. 2020 May 5;12:3131–3144. doi: 10.2147/CMAR.S243869

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© 2020 Yang et al.

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LINC00173 knockdown restricts the proliferation, migration, and invasiveness but induces the apoptosis of A375 and HT144 cells. (A) LINC00173 expression was evaluated in A375 and HT144 cells through RT-qPCR analysis following transfection of either si-LINC00173 or si-NC. (B) CCK-8 assay was performed to analyze the proliferation of A375 and HT144 cells after LINC00173 silencing. (C) The percentage of apoptotic A375 and HT144 cells transfected with either si-LINC00173 or si-NC was determined by flow cytometry. (D) The cell cycle status of LINC00173-deficient A375 and HT144 cells was determined by flow cytometry. (E) Western blotting was conducted to detect the expression levels of apoptotic-associated proteins (Bcl-2, BAX and Bcl-XL) and cell cycle-associated proteins (CDK-2, CDK-4 and cyclin D1) in A375 and HT144 cells after LINC00173 knockdown. (F and G) Migratory and invasive abilities were determined in LINC00173-deficient A375 and HT144 cells by migration and invasion assays. *P < 0.05 and **P < 0.01.