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. 2020 May 5;13:3789–3800. doi: 10.2147/OTT.S245346

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© 2020 Huang et al.

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miR-145 negatively regulates Th9 cell polarization in vitro (n=3). CD4+ T cells were obtained and then cultured in RPMI 1640 media containing 10% FBS. MiR-145 mimics or negative control oligonucleotide were transfected into CD4+ T cells, followed by the addition of TGF-β (2 ng/mL) and IL-4 (10 ng/mL). (A, B) After 72 h, relative expressions of miR-145 and IL-9 mRNA were measured by RT-PCR. (C, D) Th9 cells were analyzed by flow cytometry. (E) ELISA was used to detect IL-9 levels in the culture supernatant.

Notes: The data represent the mean ± standard deviation (SD) from three independent experiments. *P<0.05; ***P<0.01.

Abbreviations: RPMI, Roswell Park Memorial Institute; FBS, fetal bovine serum; NC, negative control; TGF-β, transforming growth factor-β; IL, interleukin; RT-PCR, reverse transcription-polymerase chain reaction; ELISA, enzyme linked immunosorbent assay.