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. 2020 May 5;13:3789–3800. doi: 10.2147/OTT.S245346

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© 2020 Huang et al.

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miR-145 inhibits HIF-1α expression in vitro (n=3). CD4+ T cells were obtained and transfected with miR-145 mimics or negative control oligonucleotide, followed by incubation for 3 days in the presence of TGF-β (2 ng/mL) and IL-4 (10 ng/mL). (A) HIF-1α mRNA was measured by RT-PCR. (B) Immunofluorescence was used to detect the expression of HIF-1α (Bar = 10 um).

Notes: The data represent the mean ± standard deviation (SD) from three independent experiments. *P<0.05.

Abbreviations: HIF-1α, hypoxia-inducible factor-1α; DAPI, 4ʹ,6-diamidino-2-phenylindole; NC, negative control; TGF-β, transforming growth factor-β; IL, interleukin; RT-PCR, reverse transcription-polymerase chain reaction.