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. 2020 May 5;13:3789–3800. doi: 10.2147/OTT.S245346

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© 2020 Huang et al.

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miR-145 inhibits Th9 cell production in a mouse model of MA (n=5). Construction of a mouse mode of MA in liver cancer, and intravenous injection of mmu-miR-145-5pagomir or negative control oligonucleotide. After 2 weeks, mice were sacrificed, and spleen tissue was isolated. (A, B) relative expressions of miR-145 and IL-9 mRNA were measured by RT-PCR. (C, D) Th9 cells were analyzed by flow cytometry. (E) ELISA was used to detect IL-9 levels. (F) The relationship between miR-145 and Th9 cells. *P<0.05; ***P<0.01.

Abbreviations: MA, malignant ascites; NC, negative control; IL, interleukin; CD4, T helper cells; RT-PCR, reverse transcription-polymerase chain reaction; ELISA, enzyme linked immunosorbent assay.