Table 1.
List of Primers Used in This Study
| Specificity | Primers | Sequence (5ʹ→3ʹ) | Annealing T (°C) |
Amplicon Length (bp) |
Target Gene | Genome Copy Number |
Reference |
|---|---|---|---|---|---|---|---|
| L. casei | LcasF LcasR |
TTTGAGGGGACGACCCTCAAGCA CGCCGACAAGCTATGAATTCACTTG |
55 | 339 | 16S-23S spacer region | 3 | Our study |
| L. paracasei | PC2a CPRrev |
GGATTGGGTTTTGCGTGATGGTCGC TGCATTTCCCCGCTTTCATGACT |
68 | 261 | mutL | 1 | 7 |
| L. rhamnosus | LrhamF LrhamR |
GGACAGGTAGAAAGTCAAACGA GCTGACCGTAAACGCAATCTTAG |
65 | 186 | mutL | 1 | 8 |
| L. plantarum | Lp-F Lp-R |
AAAATCATGCGTGCGGGTAC ATGTTGCGTTGGCTTCGTCT |
55 | 210 | pyrG | 1 | 9 |
| L. reuteri | Lreu-1 Lreu-4 |
CAGACAATCTTTGATTGTTTAG GCTTGTTGGTTTGGGCTCTTC |
60 | 305 | 16S-23S spacer region | 6 | 10 |
| L. acidophilus | LacidoF LacidoR |
TGCAAAGTGGTAGCGTAAGC CCTTTCCCTCACGGTACTG | 47 | 207 | 16S-23S spacer region | 4 | 11 |
| L. fermentum | Lfer-3 Lfer-4 |
ACTAACTTGACTGATCTACGA TTCACTGCTCAAGTAATCATC |
50 | 191 | 16S-23S spacer region | 5 | 11 |