Table 4.

Intra-Assay Repeatability, Inter-Assay Reproducibility and Detection Limits of qPCR

Target	Quantities (log10 CFU/gram)	Intra-Assay Repeatability (Ct)			Inter-Assay Reproducibility (Ct)			Detection Limit (log10 CFU/g feces)
		Mean	SD	CV %	Mean	SD	CV %
L. plantarum	4.75	19.57	0.06	0.31	19.55	0.02	0.10	3.86
L. paracasei	4.36	22.49	0.04	0.18	22.49	0.02	0.09	3.75
L. rhamnosus	4.31	22.65	0.05	0.22	22.61	0.02	0.09	3.77
L. reuteri	4.63	22.89	0.02	0.09	22.90	0.02	0.09	2.84
L. casei	4.39	22.47	0.02	0.09	22.48	0.02	0.09	3.80
L. fermentum	4.67	21.44	0.04	0.19	21.45	0.02	0.09	3.00
L. acidophilus	4.21	22.92	0.05	0.22	22.94	0.02	0.09	3.43

Abbreviations: SD, standard deviation; CV, coefficients of variation.