REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-Cyclooxygenase 2 (COX-2)	Cayman	Cat# 160106; RRID:AB_10077935
Rabbit polyclonal anti-Progesterone Receptor (PGR)	Dako	Cat# A0098; RRID:AB_2315192
Mouse monoclonal H2AX (pS139) Mouse, Alexa Fluor 647	BD Biosciences	Cat# 560447; RRID:AB_1645414
Anti-NF-κB p65 (acetyl K310) antibody - ChIP Grade	Abcam	Cat# ab19870; RRID:AB_776753
Rabbit polyclonal Anti-beta Actin	Abcam	Cat# ab8227; RRID:AB_2305186
Rabbit polyclonal anti-GAPDH	Abcam	Cat# ab9485; RRID:AB_307275
Rabbit polyclonal anti-alpha Tubulin	Abcam	Cat# ab4074; RRID:AB_2288001
Rat monoclonal anti-Neutrophil	Abcam	Cat# ab2557; RRID:AB_303154
Rabbit polyclonal anti-Neutrophil Elastase	Abcam	Cat# ab68672; RRID:AB_1658868
Goat polyclonal anti-LYVE1	R&D Systems	Cat# AF2125; RRID:AB_2297188
Rabbit polyclonal anti-Malondialdehyde (MDA)	Abcam	Cat# ab6463; RRID:AB_305484
Rat Anti-CD16 / CD32 Monoclonal (Fc Block)	BD Biosciences	Cat# 553142; RRID:AB_394657
Rat Anti-CD45 Monoclonal, PE-Cy7 Conjugated	BD Biosciences	Cat# 552848; RRID:AB_394489
Rat Anti-CD11b Monoclonal, APC-Cy7 Conjugated	BD Biosciences	Cat# 557657; RRID:AB_396772
CD11c Monoclonal (N418), PE-Cyanine5	eBioscience	Cat# 15-0114-82; RRID:AB_468717
FITC anti-mouse I-A/I-E	Biolegend	Cat# 107606; RRID:AB_313321
Rat Anti-Mouse Ly-6G Monoclonal, V450 Conjugated	BD Biosciences	Cat# 560603; RRID:AB_1727564
Brilliant Violet 421 anti-mouse Ly-6C	Biolegend	Cat# 128031; RRID:AB_2562177
PE anti-mouse/human CD45R/B220	Biolegend	Cat# 103207; RRID:AB_312992
APC Rat Anti-Mouse CD8a	BD Biosciences	Cat# 553035; RRID:AB_398527
Goat anit-rabbit, HRP-conjugated	Vector Loboratories	Cat# PK-6101; RRID:AB_2336820
Donkey anit-rat, HRP-conjugated	Jackson Immunoresearch	Cat# 712-035-150; RRID:AB_2340638
Donkey anit-rabbit, AP-conjugated	Jackson Immunoresearch	Cat# 711-055-152; RRID:AB_2340591
Chemicals, Peptides, and Recombinant Proteins
Normal Donkey Serum	Jackson Immunoresearch	Cat# 017-000-121
Pregnant Mare Serum Gonadotropin	Sigma-Aldrich	Cat# G4877
Human chorionic gonadotropin	Sigma-Aldrich	Cat# CG10
M199 culture media	Life Technologies, Inc.	Cat# 12350–039
DMEM/F-12, HEPES	GIBCO	Cat# 11330–032
ITS Liquid Media Supplement (100 ×)	Sigma-Aldrich	Cat# I3146
Penicillin-Streptomycin (10,000 U/mL)	GIBCO	Cat# 15140–122
Sodium bicarbonate	Sigma-Aldrich	Cat# S5761
Sucrose	Sigma-Aldrich	Cat# S0389
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid	Sigma-Aldrich	Cat# E3889
Fetal Bovine Serum	Sigma-Aldrich	Cat# F2442
Collagenase type I	Invitrogen	Cat# 17100–017
Deoxyribonuclease I	Sigma-Aldrich	Cat# D4527
Bovine serum albumin	Sigma-Aldrich	Cat# A9418
DAB Peroxidase (HRP) Substrate Kit (with Nickel), 3,3′-diaminobenzidine	Vector Loboratories	Cat# SK-4100
Trichostatin A	Sigma-Aldrich	Cat# T8552
Nicotinamide	Sigma-Aldrich	Cat# 72340
RU486 (mifepristone)	Cayman	Cat# 10006317
Fetal Bovine Serum Stain Buffer	BD Biosciences	Cat# 554656
Benzene	Sigma-Aldrich	Cat# 270709
Ethyl acetate	Sigma-Aldrich	Cat# 270989
Methanol	Sigma-Aldrich	Cat# 34860
GelRed® Nucleic Acid Gel Stain	Biotium	Cat# 41003
Critical Commercial Assays
Single-Cell 3′ Chromium kit V3	10X Genomics	Cat# PN-1000092
Chromatin immunoprecipitation (ChIP) assays kit	Abcam	Cat# ab500
PGE2 enzyme immunoassay (EIA) kit	Cayman	Cat# 514010
Deposited Data
Mouse ovary single-cell RNA-seq dataset	This paper	GEO: GSE145107
Mouse granulosa cell ChIP-seq data	Dinh et al., 2019	GEO: GSE115820
Experimental Models: Organisms/Strains
Mouse: Pgrflox/flox, C57BL/6 background	Fernandez-Valdivia et al., 2010	N/A
Mouse: Esr2iCre/iCre, C57BL/6 background	In house, available from Jackson laboratory	JAX stock #028717
Mouse: Zp3Cre/Wt, C57BL/6 background	Jackson laboratory	JAX stock #003651
Oligonucleotides
PCR primers	Table S2	N/A
Software and Algorithms
Cell Ranger 3.0.2	10x Genomics	https://support.10xgenomics.com/single-cell-gene-expression/software/release-notes/3–0
R (v3.5)	R Core Team, 2013	https://www.r-project.org/
Seurat R package (v3.0.0)	Satija et al., 2015	https://satijalab.org/seurat/
Monocle R package (v2.10.1)	Trapnell et al., 2014	http://cole-trapnell-lab.github.io/monocle-release/
Uniform Manifold Approximation and Projection (UMAP)	McInnes et al., 2018
Hamamatsu NDP viewer V2	Hamamatsu Photonics	https://www.hamamatsu.com/us/en/product/type/U12388-01/index.html
ImageJ	Schneider et al., 2012	https://imagej.nih.gov/ij/plugins/cell-counter.html
FCS Express 6	De Novo Software	https://denovosoftware.com/
GraphPad Prism	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
SPSS version 18.0	SPSS Inc.	https://www.ibm.com/analytics/spss-statistics-software