| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-Cyclooxygenase 2 (COX-2) | Cayman | Cat# 160106; RRID:AB_10077935 |
| Rabbit polyclonal anti-Progesterone Receptor (PGR) | Dako | Cat# A0098; RRID:AB_2315192 |
| Mouse monoclonal H2AX (pS139) Mouse, Alexa Fluor 647 | BD Biosciences | Cat# 560447; RRID:AB_1645414 |
| Anti-NF-κB p65 (acetyl K310) antibody - ChIP Grade | Abcam | Cat# ab19870; RRID:AB_776753 |
| Rabbit polyclonal Anti-beta Actin | Abcam | Cat# ab8227; RRID:AB_2305186 |
| Rabbit polyclonal anti-GAPDH | Abcam | Cat# ab9485; RRID:AB_307275 |
| Rabbit polyclonal anti-alpha Tubulin | Abcam | Cat# ab4074; RRID:AB_2288001 |
| Rat monoclonal anti-Neutrophil | Abcam | Cat# ab2557; RRID:AB_303154 |
| Rabbit polyclonal anti-Neutrophil Elastase | Abcam | Cat# ab68672; RRID:AB_1658868 |
| Goat polyclonal anti-LYVE1 | R&D Systems | Cat# AF2125; RRID:AB_2297188 |
| Rabbit polyclonal anti-Malondialdehyde (MDA) | Abcam | Cat# ab6463; RRID:AB_305484 |
| Rat Anti-CD16 / CD32 Monoclonal (Fc Block) | BD Biosciences | Cat# 553142; RRID:AB_394657 |
| Rat Anti-CD45 Monoclonal, PE-Cy7 Conjugated | BD Biosciences | Cat# 552848; RRID:AB_394489 |
| Rat Anti-CD11b Monoclonal, APC-Cy7 Conjugated | BD Biosciences | Cat# 557657; RRID:AB_396772 |
| CD11c Monoclonal (N418), PE-Cyanine5 | eBioscience | Cat# 15-0114-82; RRID:AB_468717 |
| FITC anti-mouse I-A/I-E | Biolegend | Cat# 107606; RRID:AB_313321 |
| Rat Anti-Mouse Ly-6G Monoclonal, V450 Conjugated | BD Biosciences | Cat# 560603; RRID:AB_1727564 |
| Brilliant Violet 421 anti-mouse Ly-6C | Biolegend | Cat# 128031; RRID:AB_2562177 |
| PE anti-mouse/human CD45R/B220 | Biolegend | Cat# 103207; RRID:AB_312992 |
| APC Rat Anti-Mouse CD8a | BD Biosciences | Cat# 553035; RRID:AB_398527 |
| Goat anit-rabbit, HRP-conjugated | Vector Loboratories | Cat# PK-6101; RRID:AB_2336820 |
| Donkey anit-rat, HRP-conjugated | Jackson Immunoresearch | Cat# 712-035-150; RRID:AB_2340638 |
| Donkey anit-rabbit, AP-conjugated | Jackson Immunoresearch | Cat# 711-055-152; RRID:AB_2340591 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Normal Donkey Serum | Jackson Immunoresearch | Cat# 017-000-121 |
| Pregnant Mare Serum Gonadotropin | Sigma-Aldrich | Cat# G4877 |
| Human chorionic gonadotropin | Sigma-Aldrich | Cat# CG10 |
| M199 culture media | Life Technologies, Inc. | Cat# 12350–039 |
| DMEM/F-12, HEPES | GIBCO | Cat# 11330–032 |
| ITS Liquid Media Supplement (100 ×) | Sigma-Aldrich | Cat# I3146 |
| Penicillin-Streptomycin (10,000 U/mL) | GIBCO | Cat# 15140–122 |
| Sodium bicarbonate | Sigma-Aldrich | Cat# S5761 |
| Sucrose | Sigma-Aldrich | Cat# S0389 |
| Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid | Sigma-Aldrich | Cat# E3889 |
| Fetal Bovine Serum | Sigma-Aldrich | Cat# F2442 |
| Collagenase type I | Invitrogen | Cat# 17100–017 |
| Deoxyribonuclease I | Sigma-Aldrich | Cat# D4527 |
| Bovine serum albumin | Sigma-Aldrich | Cat# A9418 |
| DAB Peroxidase (HRP) Substrate Kit (with Nickel), 3,3′-diaminobenzidine | Vector Loboratories | Cat# SK-4100 |
| Trichostatin A | Sigma-Aldrich | Cat# T8552 |
| Nicotinamide | Sigma-Aldrich | Cat# 72340 |
| RU486 (mifepristone) | Cayman | Cat# 10006317 |
| Fetal Bovine Serum Stain Buffer | BD Biosciences | Cat# 554656 |
| Benzene | Sigma-Aldrich | Cat# 270709 |
| Ethyl acetate | Sigma-Aldrich | Cat# 270989 |
| Methanol | Sigma-Aldrich | Cat# 34860 |
| GelRed® Nucleic Acid Gel Stain | Biotium | Cat# 41003 |
| Critical Commercial Assays | ||
| Single-Cell 3′ Chromium kit V3 | 10X Genomics | Cat# PN-1000092 |
| Chromatin immunoprecipitation (ChIP) assays kit | Abcam | Cat# ab500 |
| PGE2 enzyme immunoassay (EIA) kit | Cayman | Cat# 514010 |
| Deposited Data | ||
| Mouse ovary single-cell RNA-seq dataset | This paper | GEO: GSE145107 |
| Mouse granulosa cell ChIP-seq data | Dinh et al., 2019 | GEO: GSE115820 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Pgrflox/flox, C57BL/6 background | Fernandez-Valdivia et al., 2010 | N/A |
| Mouse: Esr2iCre/iCre, C57BL/6 background | In house, available from Jackson laboratory | JAX stock #028717 |
| Mouse: Zp3Cre/Wt, C57BL/6 background | Jackson laboratory | JAX stock #003651 |
| Oligonucleotides | ||
| PCR primers | Table S2 | N/A |
| Software and Algorithms | ||
| Cell Ranger 3.0.2 | 10x Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/release-notes/3–0 |
| R (v3.5) | R Core Team, 2013 | https://www.r-project.org/ |
| Seurat R package (v3.0.0) | Satija et al., 2015 | https://satijalab.org/seurat/ |
| Monocle R package (v2.10.1) | Trapnell et al., 2014 | http://cole-trapnell-lab.github.io/monocle-release/ |
| Uniform Manifold Approximation and Projection (UMAP) | McInnes et al., 2018 | |
| Hamamatsu NDP viewer V2 | Hamamatsu Photonics | https://www.hamamatsu.com/us/en/product/type/U12388-01/index.html |
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/plugins/cell-counter.html |
| FCS Express 6 | De Novo Software | https://denovosoftware.com/ |
| GraphPad Prism | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| SPSS version 18.0 | SPSS Inc. | https://www.ibm.com/analytics/spss-statistics-software |
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