Figure 3. HSPC mobilization is associated with CD34 asymmetric inheritance and cell differentiation.

(A) The percentage of CD34-positive cells in cultured trapped and escaped HSPC groups was monitored over time by flow cytometry. Data are presented as mean ± s.d. from three different experiments (n=3, measured three times, for each group). *P < 0.01 determined by a two-tailed Student’s t test. (B) CD34 staining of dividing HSPCs was performed to monitor for CD34 asymmetric inheritance. The bottom panel demonstrates the measurement of fluorescence intensity along the membrane contour of dividing cells, with distance normalized relative to total cell length. Scale bar: 2 μm. (C) Rates of CD34 asymmetric inheritance in the trapped and escaped cell groups were determined by monitoring CD34 staining of dividing cells. For each experiment, equal number of trapped and escaped cells (n=30) was analyzed. Data are presented as mean ± s.d. from three different experiments (n=3, measured three times, for each group). *P < 0.01 determined by a two-tailed Student’s t test. (D) Fold-change (log₂) in expression of homeostasis regulation genes in the escaped relative to trapped cells was determined using RNA-seq analysis.