Fig. 3.

Agarose gel electrophoresis (1% agarose) of polymerase chain reaction (PCR) amplified products using HPV16 E6-specific primer sets. Gel film depicts results of samples obtained from the operating room of a p16-positive tumor. The positive control contained 1.5 μg of DNA. The negative control consisted of a Tris and ethylenediaminetetraacetic acid buffer. PCR was considered tentatively positive if a band of the expected size (approximately 320 bp for E6) was visible.