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. 2020 Apr 20;117(18):9964–9972. doi: 10.1073/pnas.1913633117

Fig. 2.

Fig. 2.

Enhanced Nrf2-driven pathway activity in IDH1-mutated cells. (A) Relative luciferase activity of Dox-induced IDH1-mutated U251 cells transfected with the pGL4.37 ARE-luciferase reporter vector. The luciferase activity was normalized by Renilla luciferase. n = 3. **P < 0.01. (B) Nrf2 activity was determined using a Cignal ARE-luciferase reporter assay in BTIC lines. n = 3. **P < 0.01. (C) Promoter affinity of Nrf2 was measured by ChIP-PCR in U251 WT, R132C, and R132H cells. n = 3. **P < 0.01, *P < 0.05. (D) Gene expression assay quantified ROS generating and scavenging genes in BTIC lines. (E) qRT-PCR analysis of Nrf2-associated expression in BTIC lines. Each gene was normalized to its control. n = 3. (F) Immunoblotting of SLC7A11 expression in BTIC and Dox-induced IDH1-mutated U251 cells. β-actin was used as an internal control.