Skip to main content
. 2020 Apr 20;117(18):9964–9972. doi: 10.1073/pnas.1913633117

Fig. 3.

Fig. 3.

SLC7A11 plays a key role in the redox balance and survival of IDH1-mutated cells. (A) GSH/GSSG ratio was measured using GSH/GSSG-Glo assay in TS603 cells with siRNA targeting SLC7A11. n = 3. **P < 0.01. (B) Lipid peroxidation assay of TS603 cells with siRNA targeting SLC7A11. Before lipid peroxidation, the emission fluorescence showed at 590 nm (red). After lipid peroxidation, the emission fluorescence showed at 510 nm (green). (C) Lipid peroxidation was quantified using the ratio of F510 nm to F590 nm. n = 5. **P < 0.01. (D) ROS-Glo H2O2 assay of TS603 cells with siRNA targeting SLC7A11. (E) Annexin V/PI apoptosis assay of BTIC lines (IDH1WT GSC827 and IDH1R132H TS603 cells) with siRNA targeting SLC7A11. (F) Quantification of apoptosis assay. n = 3. **P < 0.01.