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. Author manuscript; available in PMC: 2020 May 11.
Published in final edited form as: Science. 2019 Sep 27;365(6460):1466–1469. doi: 10.1126/science.aav7321

Fig. 4. Chromatin threads in human meiosis.

Fig. 4

(A) MII chromosomes with prominent gaps aligned correctly on the spindle. Arrow points to a prominent gap between sister chromatids (gray: DNA, magenta: centromeres marked by CREST serum, green: microtubules). Scale bars: 4μm (overview) and 2μm (inset).

(B and C) Proportion of oocytes having at least one chromosome with a pronounced gap (B) and quantitative analysis of separation of the two chromatids across the entire oocyte cohort (C).

(D) Chromatin threads between chromatids with weakened centromeric cohesion. All chromosomes captured prior to anaphase onset. Scale bars: 2μm (overview) and 0.5μm (insets).

(E) Proportion of MII oocytes with chromatin threads by donor age grouped into above and below our AMAs cut-off. Exact test: p<0.05 (*). All analysis performed on intact spindles.

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