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. 2020 Mar 22;34(5):6284–6301. doi: 10.1096/fj.201903051R

Figure 6.

Figure 6

AMPK activation is responsible for MFF phosphorylation at Ser 146 in skeletal muscle cells. A, 991 treatment induces MFF phosphorylation in skeletal muscle cells. C2C12 myotubes were serum starved for 4 h prior to the treatment of DMSO (0.1%) as a vehicle control (CTRL) or AMPK activator 991 at the indicated concentrations for 2 h. B, CCCP treatment induces MFF phosphorylation. C2C12 myotubes were treated with DMSO (0.1%, 24 h) as a vehicle control (CTRL) or 10 μM of CCCP for up to 24 h. C, 991‐induced MFF phosphorylation is diminished in AMPK ⍺1/⍺2 deficient cells. HEK293 wild type and AMPK ⍺1/⍺2 deficient cells were treated with DMSO (0.1%) as a vehicle control (CTRL) or AMPK activator 991 (10 μM) for 1 h and 2 h. Cells were serum starved for 2 h prior to treatment. Total lysates were analyzed by SDS‐PAGE and western blotting with the indicated antibodies. Representative images of n = 3 independent experiments are shown. Data are expressed as means ± SEM; *P < .05, **P < .01, ***P < .001, ****P < .0001 compared to CTRL