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. Author manuscript; available in PMC: 2020 May 11.
Published in final edited form as: Sci Transl Med. 2019 Sep 18;11(510):eaav5055. doi: 10.1126/scitranslmed.aav5055

Fig. 6. The pro-oxidant effects of WNT5A in human vascular smooth muscle cells are mediated by Frizzled 2 (Fzd2) and Frizzled 5 (Fzd5) receptors.

Fig. 6

(A) Fluorescence lipofectamine RNAimax imaging (BLOCK-iT Alexa Fluor Red Fluorescence) against cell nuclei stained with DAPI (blue signal), (B) fexpression old change, and (C) Western blotting of transfection efficiency of knock-down of FZD2 and FZD5 genes in VSMCs (~96% downregulation of FZD2, ~65% downregulation of FZD5; n = 3). (D) Basal, (E) NADPH-stimulated, and (F) Vas2870-inhibitable O2.- production in siRNA-FZD2-treated VSMCs (n = 5-6 pairs per intervention) in the presence or absence of WNT5A. (G) Basal, (H) NADPH-stimulated, and (I) Vas2870-inhibitable O2.- production in siRNA-FZD5-treated VSMCs (n = 6 pairs per intervention) in the presence or absence of WNT5A. Data are presented as mean ± SEM. *P < 0.05 vs control by Wilcoxon signed ranks tests (D-I) or paired t-test (B). SEM: Standard error of the mean.