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. Author manuscript; available in PMC: 2020 May 11.
Published in final edited form as: Sci Transl Med. 2019 Sep 18;11(510):eaav5055. doi: 10.1126/scitranslmed.aav5055

Fig. 8. USP17 as a link between WNT5A and vascular redox signaling.

Fig. 8

(A) Migration of VSMCs incubated with peg-SOD, an intracellular scavenger of superoxide (O2.- ) production, (n = 5-8 pairs per intervention) and treated with WNT5A. (B) A subset of the WNT5A cell-motility differentially expressed genes of the microarray analysis were at least partially rescued by superoxide (O2.- ) anion scavenging with superoxide dismutase (peg-SOD) resulting in p-values>0.05 (n = 5 pairs, genes presented by descending mean fold change). (C) USP17 expression in VSMCs incubated with peg-SOD and treated with WNT5A (n = 10). (D) RAC1 activation in HeLa cells transfected with shUSP17 and treated with WNT5A (n = 8). Data are presented as mean ± SEM in panels A, C and D. *P < 0.05 vs control by Wilcoxon signed rank test in panels A & C.+P<0.05 vs untreated empty vector control by Wilcoxon signed rank test; NS by Wilcoxon signed rank test for WNT5A -treated vs untreated shUSP17 cells in panel D.